rabbit anti cd19 fitc conjugated antibody  (Bioss)

Journal: Microbiology Spectrum

Article Title: Streptococcus suis Serotype 2 Infection Induces Splenomegaly with Splenocyte Apoptosis

doi: 10.1128/spectrum.03210-22

Figure Lengend Snippet: Identification of apoptotic cells and apoptosis pathway in the spleens of infected mice. (A) Appropriate FITC-conjugated antibodies were used to label CD19 + cells. Apoptotic cells (red) were detected by TUNEL assay, and nuclei (blue) were stained with 4′,6-diamidino-2-phenylindole (DAPI); scale bars represent 20 μm. (B) Number of apoptotic B cells in spleens from infected and control groups of mice. (C) Using confocal laser scanning microscopy, cells were stained for caspase-3 antibody (red). B cells were stained for CD19 + antibody (green), and nuclei were stained by DAPI (blue); scale bars represent 20 μm. (D) Levels of caspase-3, caspase-8, and caspase-9 proteins in the spleens of mice at 1, 2, 4, and 7 dpi. Spleen tissues were lysed, and Western blotting was performed. (E) Using confocal laser scanning microscopy, cells were stained for AIF antibody (green). Apoptotic cells (red) were detected by TUNEL assay, and cell nuclei were stained by DAPI (blue); scale bars represent 20 μm.

Article Snippet: To confirm the types of cells that underwent apoptosis or pyroptosis, spleen sections were stained with different antibodies, including FITC-conjugated rabbit anti-mouse CD3, CD19 antibody (1:50, Southern Biotech), Alexa Fluor 647 rabbit anti-mouse F4/80 antibody (1:50; Abcam, Cambridge, UK), rabbit anti-mouse caspase-3, gasdermin D-N (GSDMD-N) antibody (1:300; Cell Signaling, Danvers, MA, USA), and Alexa Fluor 568-conjugated goat anti-rabbit antibody (1:500; Sigma, Saint Louis, MO, USA).

Techniques: Infection, TUNEL Assay, Staining, Control, Confocal Laser Scanning Microscopy, Western Blot

Journal: Microbiology Spectrum

Article Title: Streptococcus suis Serotype 2 Infection Induces Splenomegaly with Splenocyte Apoptosis

doi: 10.1128/spectrum.03210-22

Figure Lengend Snippet: Activation of inflammation signaling pathway and identification of pyrolyzed cells in mouse spleen by S. suis infection. (A) Levels of TLR2, NLRP3, ASC, caspase-1, GSDMD-C, IL-1β, and IL-18 in the mouse spleen at 1, 2, 4, and 7 dpi. Spleen tissues were lysed, and Western blotting was performed. (B) Identification of pyrolyzed cells in mouse spleen by confocal microscopy. Macrophages were stained for F4/80 antibody (purple) and GSDMD-N antibody (red). B cells were stained for CD19 + antibody (green), and nuclei were stained by DAPI (blue); scale bars represent 20 μm. ELISA kits were used to detect levels (picograms per milliliter) of IL-6 (C), IFN-β (D), and TNF-α (E) at 12 hpi, 1, 2, 4, 7, and 10 dpi in spleens. The red squares represent cytokine levels of infected mice, and the black triangles represent cytokine levels of control mice. (F) Levels of p-JNK, p-p38, and NF-κB proteins in the spleen of mice at 1, 2, 4, and 7 dpi. Results are expressed as means ± SD, and significance was determined using one-way ANOVA and the Tukey multiple-comparison test. ***, P < 0.001; **, P < 0.01; n.s., not significant.

Article Snippet: To confirm the types of cells that underwent apoptosis or pyroptosis, spleen sections were stained with different antibodies, including FITC-conjugated rabbit anti-mouse CD3, CD19 antibody (1:50, Southern Biotech), Alexa Fluor 647 rabbit anti-mouse F4/80 antibody (1:50; Abcam, Cambridge, UK), rabbit anti-mouse caspase-3, gasdermin D-N (GSDMD-N) antibody (1:300; Cell Signaling, Danvers, MA, USA), and Alexa Fluor 568-conjugated goat anti-rabbit antibody (1:500; Sigma, Saint Louis, MO, USA).

Techniques: Activation Assay, Infection, Western Blot, Confocal Microscopy, Staining, Enzyme-linked Immunosorbent Assay, Control, Comparison